Composite

Part:BBa_K2212006:Design

Designed by: Yuya Zhao   Group: iGEM17_UC_San_Diego   (2017-10-20)


GolS+RafS+GLA+LAI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4234
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4123
    Illegal BamHI site found at 1649
    Illegal BamHI site found at 2710
    Illegal XhoI site found at 1766
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6493
    Illegal NgoMIV site found at 6659
    Illegal AgeI site found at 41
    Illegal AgeI site found at 367
    Illegal AgeI site found at 1280
    Illegal AgeI site found at 3259
    Illegal AgeI site found at 3749
    Illegal AgeI site found at 5267
    Illegal AgeI site found at 6592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1803
    Illegal BsaI site found at 5600
    Illegal BsaI.rc site found at 1668
    Illegal BsaI.rc site found at 4088


Design Notes

The basic parts are arranged in the same sequence as that of the metabolism pathway


Source

This part is a composite of four different parts BBa_2212002, BBa_2212003, BBa_2212004, and BBa_2212005 using 3A assembly method.

References