Regulatory

Part:BBa_K2209000:Design

Designed by: Qinyu Ge   Group: iGEM17_Nanjing_NFLS   (2017-10-17)


Modified promoter of aniline dioxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Protein binding to DNA which act the regulating factors in most of the gene expression. DNA sequence in promoter have consensus sequence, nucleotide in the consensus sequence mutated will largely affect the binding of regulating protein. So it could be change the level and pattern of the expression of the gene through the mutation of the promoter.



Source

P.putida and the similar strain such as pseudomonas

References