Coding

Part:BBa_K2201410:Design

Designed by: Olga Schmidt   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-27)


Kanamycin composite part containing amber codons


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 964
    Illegal NheI site found at 987
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part was constructed using overlap PCR with concluding Gibson Assembly.


Source

References