T7
RNAP amber

Part:BBa_K2201405:Design

Designed by: Olga Schmidt   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-27)


T7 RNA Polymerase with Amber Codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

This composite part was constructed using overlap PCR with concluding Gibson Assembly. The amber codon at position 1 after the start codon was introduced with primers.


Source

References