Generator

Part:BBa_K2201241:Design

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


CrtEBI under constitutive promoter with an amber codon at amino acid position 318 of crtI


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2037
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1573
    Illegal NgoMIV site found at 1703
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The amber stop codon (TAG) is inserted via site directed mutagenesis.


Source

BBa_K274110

References