Coding

Part:BBa_K2201207:Design

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Expression plasmid of an aminoacyl-tRNA synthetase for AzoPhe


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1164
    Illegal BamHI site found at 1170
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 295
    Illegal SapI.rc site found at 327
    Illegal SapI.rc site found at 1120


Design Notes

The coding sequence of the synthetase has to be inserted into a linearized ONBY-Part. For example per Gibson Assembly of a gene synthesis with matching overahangs.

It can be obtained by cloning the CDS in the linearized ONBY-part as shown in Figure 1.

Figure 1: History of the plasmid construction of K2201207.

Source

Collaboration with CU Boulder, Schultz lab, Addgene

References