Coding

Part:BBa_K2201027:Design

Designed by: Christopher M. Whitford   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


PtNTT2(31-575)-lacO-Cas9 construct


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3269
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3269
    Illegal NheI site found at 3028
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3269
    Illegal BamHI site found at 5307
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3269
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3269
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

optimized for repression and better expression rate than lacO_tight1 (K2201020).


Source

E. coli BL21(DE3)

References