DNA

Part:BBa_K2201009:Design

Designed by: Christopher M. Whitford   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Flanking sequences for deletion of codA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 580
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 580
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 580
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 580
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Both flanks are over 500 bp long


Source

The flanks were amplified via PCR from E. coli BL21(DE3).

References