Regulatory

Part:BBa_K220004:Design

Designed by: Sarah Sandock   Group: iGEM09_Wisconsin-Madison   (2009-10-18)

Salt Stress Activated Promoter Pro U


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Length: 30 Fwd: 5'- AAT ACT AGT CTT TTT TCT GCG CAC CTC ACG -3'

Length: 42 Rev: 5'- AAT ACT AGT GCA ATA GAA AGA TTC CTT TAT TTG TCT ATG TCG -3'


Source

Gene isolation from K12 E. Coli Genomic DNA


References

T Annamalai, K Venkitanarayanan

Role of proP and proU in betaine uptake by Y. enterocolitica under cold and osmotic stress conditions

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655450/?tool=pubmed"