Coding
Part:BBa_K2198004:Design
Designed by: Gabriel Dournes Group: iGEM17_Pasteur_Paris (2017-10-25)
Trans-2-carboxybenzalpyruvate hydratase-aldolase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1135
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 993
Illegal AgeI site found at 118
Illegal AgeI site found at 541
Illegal AgeI site found at 783 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence optimized for expression in E.coli and for gene synthesis. It has also a RBS, Histag, TEV site in 5' region and a BamhI site in the 3' region.
Source
Nocardioides sp. bacterial DNA.