Regulatory
hucO

Part:BBa_K2197303:Design

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-08-10)


Repressor operator site for mammalian urate-dependent transsilencer (mUTS).


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In the reference research article form ResearchGate, hucO motif originated from Deinococcus radiodurans R1 is replicated 8 times so that the chance of binding is higher. However, this design is very difficult to be synthesized chemically. Therefore, we reduce it to one tandem hucO.



Source

Deinococcus radiodurans R1


References