Regulatory
hucO

Part:BBa_K2197303

Designed by: Ip Wang Chak   Group: iGEM17_Hong_Kong_UCCKE   (2017-08-10)


Repressor operator site for mammalian urate-dependent transsilencer (mUTS).

Design of BBa_K2197300 T--Hong_Kong_UCCKE--300gene2.jpg

Expression of a strong repressor (mUTS) T--Hong_Kong_UCCKE--Krab.jpg

Operator site and GFP T--Hong_Kong_UCCKE--realbindsite.jpg

Expression without uric acid (-UA) T--Hong_Kong_UCCKE--noUA.jpg

mUTS binds with HucO and repress the expression of downstream GFP.

expression with uric acid (+UA) T--Hong_Kong_UCCKE--wiffUA.jpg

mUTS dissociates with HucO to different extents according to the concentration of uric acid, thus expressing downstream GFP at different levels.

This is a subpart of BBa_K2197300. Engineered E.coli encodes part BBa_K2197303 which is a operating site for chimeric mammalian urate-dependent transsilencer (mUTS) or KRAB-HucR protein complex. The circuit uses a bacterial transcriptional repressor (HucR) which binds to a DNA sequence motif (hucO) in the absence of uric acid. When uric acid is present, HucR dissociates from hucO motif, thereby allows an expression of a downstream gene. According to our research, the expression of the downstream gene is regulated by the concentration of uric acid. In a reference research article form ResearchGate, hucO motif originating from Deinococcus radiodurans R1 is replicated 8 times so that the chance of binding is higher. However, this design is very difficult to be synthesized chemically. Therefore, we reduce it to one tandem hucO. When this part is put in BBa_K2197300, it works with BBa_K2197302 and acts as an operator site for it.

When this part is put in a composite part, the gene's expression will be regulated by the presence of uric acid, that is, it will only be expressed if uric acid is present.

Wilkinson, S.P. & Grove, A. HucR, a novel uric acid-responsive member of the MarR family of transcriptional regulators from Deinococcus radiodurans. J. Biol. Chem. 279, 51442–51450 (2004).

Wilkinson, S.P. & Grove, A. Negative cooperativity of uric acid binding to the transcriptional regulator HucR from Deinococcus radiodurans. J. Mol. Biol. 350, 617–630 (2005).

Contribution

Group: QHFZ-China iGEM 2019
Author: Cheng Li
Design:

Figure 1. Schematic cartoon of the DNA construct of BBa_K3007000

This year we use BBa_K3007000 as hucO. The part is equal to BBa_K2197303. hucO is HucR binding sequence, however the sequence is taggtagacatctaagta. In BBa_K2197303, there is some additional bases at 5' and 3' end of the hucO sequence. As we analyzed, they are the -35 and -10 regions of a promoter.In our experiment, we use the -35 and -10 regions of lac promoter to replace them.










Documentation:
We used hucO part to construct PhucR, BBa_K3007001 (https://parts.igem.org/Part:BBa_K3007001). Then we charactered the part in the composite parts BBa_K3007029 (https://parts.igem.org/Part:BBa_K3007029) and BBa_K3007030 (https://parts.igem.org/Part:BBa_K3007030).
This year, QHFZ-China designed a UA monitor system in E. coli (Fig. 1). Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to hucO sequence (located in PhucR), which inhibits the expression of downstream reporter, dsRed or sfGFP. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from hucO, and induces the fluorescent protein expression.

Figure 1. Working mechanism of the uric acid detection system in E. coli. (A) Schematic diagram of the gene circuit design. (B) Structure of PhucR, showing the location of BBa_K3007000.


We used the process shown in Fig. 2 to test if the UA detection system works well.

Figure 2. Work flow chart for subsequent experiments


Two clones with UA detection system with dsRed as a reporter were tested. The original gene circuit was able to response to UA in a range of 0 to 200 μM (Fig. 3A), and the clone 1 showed much better dynamics than the other (Fig. 3B). Time course experiments showed that the fluorescence intensity became quite strong at 4 to 6 hours after UA induction, and became stable at 10 to 12 hours (Fig. 3C). Even if we removed UA by replacing fresh LB medium, after 48 hours shaking, the fluorescence would be still notable (Fig. 3D) and there was not significant difference of dsRed fluorescence / OD600 between before and after UA removing (Fig. 3E). All the data meant our design could detect high UA concentration quickly and stably.

Figure 3. Response of UA detection system after different concentration of UA induction. (A) A photo to visualize the fluorescence induced by UA under a blue light. (B) Responding curve about the dsRed fluorescence / OD600 to different UA concentration of two E. coli clones. Data were shown as mean ± SD. N = 3 technical repetitions. (C) Time course experiments about the dsRed fluorescence / OD600 of E. coli after 0, 20 or 100 μM UA addition. Data were shown as mean ± SD. N = 3 technical repetitions. (D) A photo to visualize the fluorescence after UA removal under a blue light. (E) Quantitative measurement of dsRed fluorescence / OD600 before and after UA removal.


We also tried more conditions to test if this system could work well in different environment. In the range of pH 6.0 to 8.0, response of the gene circuit was relatively stable (Fig. 4A). However, the volume of the reaction system would influence the response to UA (Fig. 4B). A possible explain was the relative surface area of the liquid level changed and consequently the dissolved oxygen changed. This result meant the experiments for UA detection should be done at the same reaction system volume. In other experiments, 1 mL reaction volume was used.

Figure 4. Impact of different conditions on the UA detection system. (A) Impact of pH value on dsRed fluorescence / OD600 after 20 μM UA addition. Data were shown as mean ± SD. N = 3 technical repetitions. (B) Impact of reaction volume on dsRed fluorescence / OD600 after 0 and 20 μM UA addition. Data were shown as mean + SD. N = 3 technical repetitions.


Because the designed applications included to detect UA in blood or saliva sample, which contained serum, we tested if serum affects the detection efficiency. In view of safety, commercial fetal bovine serum (FBS) (EVERY GREEN, 11011-8611) was used here. The growth of the bacteria was obviously suppressed when the volume of FBS fraction was more than 1/1000 (Fig. 5A, 5B), which meant 1 μL fetal bovine serum was added to 1000 μL final reaction system. When the volume of FBS fraction was 1/1000, the UA detection efficacy was unaffected by serum (Fig. 5C).

Figure 5. Impact of commercial FBS on the UA detection system. (A) Pictures of E. coli cultured with or without FBS. (B) Quantitative measurement of sample shown in Fig 5A. (C) Quantitative measurement of dsRed fluorescence / OD600 after 20 μM UA addition with or without FBS. Data were shown as mean + SD. N = 3 technical repetitions.


We verified the designed system did response UA in different environments. However, during our human practices, some of interviewees worried about long responding time, which needed 4 to 6 hours after UA induction to express strong fluorescence intensity. In their opinions, users would not wait for a 4-hour reaction. And there was another feedback that the standard of hyperuricemia is ≥7 mg/dL (about 400 μM) UA for men and ≥6.0 mg/dL (about 350 μM) UA for women [2]. If we want to use our system to detect a clinical sample directly, the sample should be diluted to 1/1000 before start, which means the gene circuit is required to detect 400× 1/1000 = 0.4 μM UA as a threshold. In one word, we need a modification to shorten the responding time and increase the sensitivity of the UA detector.
For the first question, we interviewed Dr. Xiaoyu Chen, a scientist who majors in biosensors. He suggested us that changing the fluorescent protein was a means to optimize the response speed. We blasted the sequence of dsRed we used in the database, and found the maturation half-time of dsRed is about 40 minutes in E. coli [3]. To shorten the maturation time, we decided to change dsRed to superfolder GFP, whose maturation half-time was only about 13 minutes [4]. To solve the second problem, we referenced a modular, cascaded signal amplifying methodology, which induces a module named amplifier, and it may increase sensitivity of the biosensor and boost the output expression [5]. We ordered two sequences of ultrasensitive phage activator RinA_p80α (from Staphylococcal aureus phage 80α) and a promoter PrinA_p80α. We introduced these new parts to design a new version of the UA detection system, called Version 2, shown in Fig. 6.
The processes in Version 2 were almost equal to the old version, except that the downstream of PhucR was RinA_p80α. This meant if UA presented, RinA_p80α would express and active transcription of sfGFP which was under control of PrinA_p80α. Theoretically, the new design would sense UA with much higher sensitivity than the old one. In the same time, the fluorescence production of Version 2 would get faster because that sfGFP had a shorter maturation time than dsRed in old version.

Figure 6. Working mechanism of Version 2 in E. coli.


We tested the sfGFP production of Version 2 under different concentration of extracellular UA. The curve in Fig. 7A showed the fluorescence was saturated under only 15 μM UA induction, while the old version needed about 100 μM UA to get saturated (Fig. 3B). This result verified the Version 2 had higher sensitivity than the old one. To test if sfGFP could shorten the reaction time, we used the same construct only except reporter genes, called PRinA_p80α – sfGFP and PRinA_p80α – dsRed, respectively. After adding 20 μM UA into the reaction system, the curve of PRinA_p80α – sfGFP climbed much faster than PRinA_p80α – dsRed, which suggested our new design had a great induction performance, and fitted our predictions very well.

Figure 7. The induction performances of the Version 2. (A) Induction curve of Version 2 under 0 to 200 μM UA treatment by measuring the sfGFP fluorescence / OD600. Data were shown as mean ± SD. N = 3 technical repetitions. (B) Time course experiment of sfGFP Version 2 and dsRed Version 2. Data were normalized by taking the fluorescence / OD600 of two groups at 0 h as standard, respectively. Data were shown as mean ± SD. N = 3 technical repetitions.



References:
[1] Wilkinson, S. P., & Grove, A. (2004). HucR, a novel uric acid-responsive member of the MarR family of transcriptional regulators from Deinococcus radiodurans. Journal of biological chemistry, 279(49), 51442-51450.
[2] de Oliveira, E. P., & Burini, R. C. (2012). High plasma uric acid concentration: causes and consequences. Diabetology & metabolic syndrome, 4(1), 12.
[3] Bevis, B. J., & Glick, B. S. (2002). Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nature biotechnology, 20(1), 83.
[4] Pédelacq, J. D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature biotechnology, 24(1), 79.
[5] Wan, X., Volpetti, F., Petrova, E., French, C., Maerkl, S. J., & Wang, B. (2019). Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals. Nature chemical biology, 15(5), 540.






































Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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