Coding

Part:BBa_K2196003:Design

Designed by: Nicholas Roig, Julianna Casella, Chloe Savino, Jerin Thomas, Megan Schiesser, and Timothy Darby   Group: iGEM17_Stony_Brook   (2017-10-23)


Lacticin Z-Aureocin A53 Hybrid


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When designing this insert, it was initially intended to be used in the pDB.His.X series plasmids and the pET-28 derived plasmid pTHST. The pDB.His.X series plasmids contain the T7 promoter, a 6xHis tag, followed by an expression tag (GST, MBP) and a TEV protease cleavage site. Expression of our insert can be induced by adding IPTG to a liquid culture of E. coli transformed with our construct. Since our construct already contained an expression tag, a promoter, and a RBS in the vector, these were not included in the design of our insert.



Source

Staphylococcus aureus A53 and Lactococcus lactis

References