Composite

Part:BBa_K2187001:Design

Designed by: Ella Coleman   Group: iGEM16_Baltimore_BioCrew   (2016-10-14)


Chlorogenate Esterase gene


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 69
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 69
    Illegal NotI site found at 75
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 69
    Illegal BamHI site found at 237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 69
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 69
    Illegal XbaI site found at 84
    Illegal NgoMIV site found at 524
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We added a lacl regulated promoter, BBa_R0011, to allow us to turn up enzyme production. In addition, we added a prefix and suffix, a BBa_B0030 RBS, and a BBa_K864600. We also had to change two internal Pst1 sites.


Source

We used the genomic sequence of DNA: GAP38911.1 from GenBank.

References