Composite

Part:BBa_K2185003:Design

Designed by: Marissa Sumathipala   Group: iGEM16_BroadRun-Baltimore   (2016-09-05)


Penicillium alpha amylase w/ no promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1522
    Illegal XhoI site found at 1545
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 114
    Illegal AgeI site found at 336
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 650
    Illegal BsaI site found at 722


Design Notes

Removed Kpn and spe sites. Codon optimized for S.cerevisiae.


Source

Coding sequence - pencilium fungus (sequence from GenBank)

References