DNA

Part:BBa_K2184038:Design

Designed by: Margalit Mualem   Group: iGEM16_Danci-K8   (2016-09-30)


mutant SNP for non taster Umami & Monosodium gluten taste with guidRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 165


Design Notes

Engineered mutant SNP for non taster Umami & Monosodium gluten taste by swich T with C base. Engineered guidRNA to this SNP using a tool called Crispr.MIT.Edu. guide wAS the same for SNP. the SNP made enough of a difference to create or destroy a PAM site. as Cas9 would cut one kind and not the other (PAM is NGG, so if one SNP had NGG and one had NGC as a SNP,We engineered a guide upstream to this PAM and expect Cas9 to cut the NGG form and not the NGC.)


Source

Engineered mutant SNP for non taster Umami & Monosodium gluten taste- Human genomic. Engineered guidRNA to this SNP using a tool called Crispr.MIT.Edu

References