Generator

Part:BBa_K218021:Design

Designed by: Emily Hicks   Group: iGEM09_Calgary   (2009-10-18)

LuxO D47A under constitutive expression of tetR repressible promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 904
    Illegal SapI.rc site found at 866


Design Notes

Plasmid containing LuxO D47A (pJAF544) was obtained from Bassler lab (Freeman and Bassler, 1999).


Source

Jeremy A. Freeman and Bonnie L. Bassler. A genetic analysis of the function of LuxO, a two-component response regulator involved in quorum sensing in Vibrio harveyi. 1999a. Molecular Microbiology. 31(2), 665-677.


References