Intermediate

Part:BBa_K218005

Designed by: Jeremy Kubik   Group: iGEM09_Calgary   (2009-10-19)

LuxOU generator with terminator upstream

Vibrio harveyi luminescence regulatory protein LuxO (GenBank accession# P0C5S5) and phosphorelay protein LuxU (GenBank accession# AAD12737) under control of TetR repressible promoter with upstream terminator. This LuxOU-B0015 construct is an intermediate in the construction of the AI-2 signalling circuit (LuxPQ-B0015-R0040-LuxOU-B0015; Part:BBa_K218006). This circuit was constructed with Part:BBa_K218003 and Part:BBa_K218004. For usage and biology of this intermediate, refer to Part:BBa_K218001. This construct allows for constitutive expression of LuxOU while insuring that the cloning of any upstream gene is sufficiently insulated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1053
    Illegal SapI.rc site found at 1015


[edit]
Categories
Parameters
None