Composite

Part:BBa_K2180004:Design

Designed by: Sofiane Safi-Stibler   Group: iGEM16_UPMC-Paris   (2016-08-05)


Promoter +FUR regulatory sequence + RBS + LacI-LVA + Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
    Illegal AgeI site found at 1165
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to improve the codon usage for B. subtilis


Source

The sequence is based on a previous IGEM part (add part) coding for LacI + LVA for E. coli

References