Composite

Part:BBa_K2173000:Design

Designed by: Kshitij Rai,Chandel Angad Singh   Group: iGEM16_IIT_Delhi   (2016-10-21)


aiiA with degradation tag expressed under Plux-lambda hybrid promoter and a double terminator + mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2011
    Illegal NheI site found at 2034
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 196
    Illegal AgeI site found at 1708
    Illegal AgeI site found at 1820
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1054


Design Notes

There were no considerations


Source

Vibrio fischeri

References