Coding

Part:BBa_K2172011:Design

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


Promoter-RBS-GST-Thrombin protease-SmCPS1-TEV-GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2654
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1415
    Illegal BamHI site found at 747
    Illegal BamHI site found at 1013
    Illegal XhoI site found at 1149
    Illegal XhoI site found at 1173
    Illegal XhoI site found at 2466
    Illegal XhoI site found at 2676
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 818
    Illegal NgoMIV site found at 2235
    Illegal NgoMIV site found at 2799
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


Design Notes

Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.



Source

SmCPS1 comes from the genome of Salvia miltiorrhiza. The other components are from the vector pGEX-KG.

References