Coding

Part:BBa_K2172009:Design

Designed by: Bowen Xiao   Group: iGEM16_CIEI-BJ   (2016-10-14)


Tac Promoter-RBS-GST-Thrombin Protease-GFP-Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 747
    Illegal XhoI site found at 1485
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 159


Design Notes

Two extra sticky ends are added to the gene when it is cloned via PCR. They are needed so that the part can be loaded onto other vectors. Site-directed mutagenesis is performed to alter the triplets identical to the sequence of restriction enzymes used to remove the part from the vector.


Source

The source of the part is the vector pGEX-KG.

References