Coding

Part:BBa_K2172001

Designed by: Xuejing Zhang   Group: iGEM16_CIEI-BJ   (2016-10-14)


-SmCPS1-TEV-GFP-

This part is composed of three parts: SmCPS1 -- a gene coding for an enzyme in Salvia miltiorrhiza -- TEV protease cleavage site and a GFP detector. TEV allows the enzyme to be extracted from other proteins, and the GFP detector allows researchers to determine whether the protein is being expressed or not easily.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1896
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 657
    Illegal BamHI site found at 255
    Illegal XhoI site found at 391
    Illegal XhoI site found at 415
    Illegal XhoI site found at 1708
    Illegal XhoI site found at 1918
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 60
    Illegal NgoMIV site found at 1477
    Illegal NgoMIV site found at 2041
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None