Device

Part:BBa_K2170211

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-10)


Secretory biotin ligase BirA (IRES_Kozak_IgKappa_BirA_StrepDEL) in RFC[10]

IgKappa secretory biotin ligase for biotinylation of intracellular proteins

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1306
    Illegal BamHI site found at 374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 149
    Illegal SapI site found at 306


[edit]
Categories
Parameters
None