Intermediate

Part:BBa_K2170132:Design

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-11)


C-terminal intermediate of eukaryotic hypoxie sensor (VEGF-p2A-PDGF_polyA) in RFC[10]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 833
    Illegal BamHI site found at 457
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 789
    Illegal NgoMIV site found at 1166
    Illegal NgoMIV site found at 1264
    Illegal AgeI site found at 1246
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1656
    Illegal BsaI.rc site found at 163
    Illegal BsaI.rc site found at 668
    Illegal BsaI.rc site found at 1228
    Illegal SapI site found at 537


Design Notes

none


Source

assembly

References