Reporter

Part:BBa_K217000:Design

Designed by: Jessamine Osborne   Group: iGEM09_Purdue   (2009-10-20)

cmv_tat_GFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 79
    Illegal NheI site found at 10
    Illegal NotI site found at 850
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 79
    Illegal BamHI site found at 66
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 79
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 728


Design Notes

No issues. The tat peptide can be directly attached to the GFP, allowing for the transport of GFP as cargo across cellular membranes.

Source

The source of this part is our own version of a sequence received from Shan Gao of Columbia University. The original part was a tat-GFP fusion protein with a bacterial promoter for use in E. coli.

References

For more general information about the part and project, see the [http://2009.igem.org/Team:Purdue Purdue University iGEM 2009 Wiki].


For a starting reference on tat, as well as its effectiveness as a fusion protein with GFP, please see:

Alexis, F. et al. (2006). Covalent Attachment of Low Molecular Weight Poly(ethylene imine) Improves Tat Peptide Mediated Gene Delivery. Advanced Materials, 18, 2174-2178.