Translational_Unit

Part:BBa_K216004:Design

Designed by: Edinburgh iGEM 2009   Group: iGEM09_Edinburgh   (2009-09-25)

Trz hybrid signal transduction protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 365
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If this part is used in an Escherichia coli chassis, to avoid interference from the native EnvZ sensor kinase, it may be best to use an envZ mutant chassis.

Source

Kindly supplied by Prof. G. Hazelbauer, and BioBricked by the University of Edinburgh iGEM team 2009.

References

Baumgartner, J.W., Kim, C., Brisette, R.E., Inouye, M., Park, C., and Hazelbauer, G.L. 1994. Transmembrane signalling by a hybrid protein: communication from the domain of chemoreceptor Trg that recognizes sugar-binding proteins to the kinase/phosphatase domain of osmosensor EnvZ. J. Bacteriol. 176, 1157-1163.