Coding

Part:BBa_K216000:Design

Designed by: Chris French   Group: iGEM09_Edinburgh   (2009-06-20)


luxB coding sequence from Photobacterium phosphoreum ATCC11060


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No special considerations. Be aware that luminescence requires both LuxA and LuxB. If LuxC, LuxD and LuxE are also supplied, the organism can generate its own substrate, but otherwise, a long chain aldehyde such as n-decanal must be added. Addition of flavin reduction LuxG may or may not increase brightness.


Source

_Photobacterium phosphoreum_ ATCC 11060 GenBank accession DQ988873

References