Coding

Part:BBa_K2155010:Design

Designed by: BingZhao Zhuo, Yue Yang, Zexun Wu   Group: iGEM16_NWPU   (2016-10-10)


BFD-CtDock


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 759
    Illegal NgoMIV site found at 1221
    Illegal AgeI site found at 223
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 130


Design Notes

Benzoylformate decarboxylase is flanked by prefix and suffix provided by iGEM.


Source

BFD is from Pseudomonas putida. CtDocS is from C.thermocellum ATCC 27405

References