Coding
Part:BBa_K215250:Design
Designed by: Alex Leone Group: iGEM09_Washington (2009-09-30)
Lpp + 1tmr OmpA + GS Linker + TEV site + NheI Site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 211
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Anything inserted into the NheI shouldn't have a start codon and gene should still be in frame.
Source
Harvard iGEM 2006: BBa_J36848. We added a GS Linker and TEV site.