Part:BBa_K215210

IPTG Inducible 1tmr OmpA Surface Display of WT Streptavidin

This part should display WT streptavidin + 6His, BBa_J36841, on the surface of the cell via the OmpA display system when induced with IPTG.

Note: in order to express this construct as a fusion protein, there is no T after this sequence and before the SpeI site (sequencing will show inconsistent).

This part doesn't show measurable activity. See [http://2009.igem.org/Team:Washington/Project/CDS#Custom_Display_Vector_with_wild-type_monomeric_streptavidin_inserted 2009 UW iGEM page] for more information.

Cytometry showing no difference in bound biotin-conjugated fluorophore for 1mM IPTG induced vs uninduced cells at different fluorophore concentrations. Conclusion: Wild-type monomeric streptavidin shows no measureable activity when expressed as a fusion protein with one trans-membrane OmpA.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 337
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 379
  Illegal AgeI site found at 430
  
• 1000
  COMPATIBLE WITH RFC[1000]