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Part:BBa_K2152004:Design

Designed by: XULIN GE   Group: iGEM16_ShanghaiTechChina_B   (2016-10-13)


Bacteriophage Phi X 174 lysis gene E with T7 and RBS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introduce a Point mutation at a XbaI restriction site.


Source

Bacteriophage Phi X 174

References

1 Hutchison C A, Sinsheimer R L. The process of infection with bacteriophage ΦX174: X. Mutations in a ΦX lysis gene. Journal of molecular biology 1966 18(3): 429-IN2.
2 Halfmann G, Götz F, Lubitz W. Expression of bacteriophage PhiX174 lysis gene E in Staphylococcus carnosus TM300.FEMS microbiology letters 1993 108(2): 139-143.