Other
Part:BBa_K2152004:Design
Designed by: XULIN GE Group: iGEM16_ShanghaiTechChina_B (2016-10-13)
Bacteriophage Phi X 174 lysis gene E with T7 and RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Introduce a Point mutation at a XbaI restriction site.
Source
Bacteriophage Phi X 174
References
1 Hutchison C A, Sinsheimer R L. The process of infection with bacteriophage ΦX174: X. Mutations in a ΦX lysis gene. Journal of molecular biology 1966 18(3): 429-IN2.
2 Halfmann G, Götz F, Lubitz W. Expression of bacteriophage PhiX174 lysis gene E in Staphylococcus carnosus TM300.FEMS microbiology letters 1993 108(2): 139-143.