![](https://parts.igem.org/images/partbypart/icon_reporter.png)
Reporter
Part:BBa_K2150022:Design
Designed by: Jianyi Huang Group: iGEM16_UCAS (2016-10-12)
pTet+RBS+T7RNAP+Ter+pT7+RBS+GFP+DT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 3488
Design Notes
Two different terminators are used to avoid repeated sequence.
Source
T7RNAP:PCR amplified from genome of BL21.
First terminator comes from BBa_B0013, while the second terminator from BBa_B0015. PTet from BBa_R0040,RBS from BBa_B0034, pT7 from BBa_K525998, GFP from BBa_E0040.