Reporter

Part:BBa_K2150016:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pT7 GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 690


Design Notes

With pT7 promoter, expression of GFP can be amplified by T7 RNA polymerase.


Source

pT7 promoter with RBS comes from BBa_K525998,GFP from BBa_E0040,DT from BBa_B0015.

References