Reporter

Part:BBa_K2150014:Design

Designed by: Jianyi Huang   Group: iGEM16_UCAS   (2016-10-12)


pTet GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 724


Design Notes

This part is used to test the sensitivity of ptet to tetR, so before experimenting with our desired gene,we used GFP to see whether ptet can be repressed by tetR.


Source

ptet from BBa_R0040,RBS from BBa_B0034,GFP from BBa_E0040, DT from BBa_B0015.

References