Part:BBa_K2150013:Design

A fusion protein combining tetX(BBa_K2150101) with GFP(BBa_E0040)

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 562
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1859
  

Design Notes

The protein linker is 3*GGGGS which provides flexibility to the fusion protein. When choosing the codon for this linker, we need to avoid repeated sequence which may cause problems when assembling these parts.

Source

The source of tetX was the same as (BBa_K2150101), the protein linker was provided by our advisor Li Hua, and the GFP was from BBa_E0040. We combined these three parts by overlap extension polymerase chain reaction (OE-PCR).