Translational_Unit

Part:BBa_K2149001:Design

Designed by: Aline Larissa Gonçalves   Group: iGEM16_USP-EEL-Brazil   (2016-10-13)


HPPD from Pseudomonas putida


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 82
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 82
    Illegal PstI site found at 964
    Illegal NgoMIV site found at 381
    Illegal NgoMIV site found at 502
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 865


Design Notes

The gene was synthesized with thirty nucleotides in its ends to do a Gibson Assembly overlap. Its design takes into consideration the RBS and the coding sequence.

Source

This part was taken from the genomic sequence from Pseudomonas putida (access number K00457) that is a gram-negative, rod-shaped, saprotrophic soil bacterium. This gene is involved in the carotenoids pathway and was courtesy as a part of an operon that produces tocotrienol by Dr. Albermann.

References

ALBERMANN, C. et al. Biosynthesis of the vitamin E compound delta-tocotrienol in tecombinant Escherichia coli cells. ChemBioChem, v. 9, n. 15, p. 2524–2533, 2008.