RNA
Part:BBa_K2148014:Design
Designed by: Ciara McCarthy Group: iGEM16_Cambridge-JIC (2016-10-12)
gRNA for CGG PAM
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
2 Gs added after TSS to stabilise gRNA, following advice of N. Patron (Earlham Institute)
Source
Target sequence designed using Benchling software (https://benchling.com/crispr), scaffold sequence from Addgene (https://www.addgene.org/static/cms/files/hCRISPR_gRNA_Synthesis.pdf)