Reporter
GG101

Part:BBa_K2145126:Design

Designed by: Katie Maruna, Laura Chen, Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-19)


GFP/RFP reporter (Forward/Reverse) with random 500bp spacer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 216
    Illegal AgeI site found at 328
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This plasmid was assembled using Golden Gate Assembly


Source

The RFP part is from BBa_J04450 from the iGEM registry and the GFP part is from BBa_I13522 from the iGEM registry. The backbone is a pSB1C3 backbone and there is a spacer between the RFP part and GFP part derived from different plasmid backbones.

References