Deleted
Sample Discontinued
Experience: None
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Reporter

Part:BBa_K2145003:Design

Designed by: Melody Wu   Group: iGEM16_Alverno_CA   (2016-10-14)


GFP/RFP reporter with spacer (500bp)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1621
    Illegal AgeI site found at 1733
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 213


Design Notes

Constructed with Golden Gate Assembly


Source

Constructed from BBa_J04450, BBa_I13522, and a spacer derived from a concatenation of several non-functional plasmid backbone sequences.


References