Coding

Part:BBa_K2144001:Design

Designed by: Oskar hman   Group: iGEM16_Stockholm   (2016-09-22)


Coding sequence for trunctated Lysostaphin regulated by T7-promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 540
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The subunits were aligned by 3A Assembly.


Source

The sequence encoding for lysostaphin is synthesised de novo according to iGEM Harbin 2012.

References

[1] Kokai-Kun JF, Walsh SM, Chanturiya T, Mond JJ (May 2003). "Lysostaphin cream eradicates Staphylococcus aureus nasal colonization in a cotton rat model". Antimicrobial Agents and Chemotherapy.

[2] Wu JA, Kusuma C, Mond JJ, Kokai-Kun JF (Nov 2003). "Lysostaphin disrupts Staphylococcus aureus and Staphylococcus epidermidis biofilms on artificial surfaces".Antimicrobial Agents and Chemotherapy.

[3] Paul A. Recsei et al, 1987, "Cloning, sequence, and expression of the lysostaphin gene from Staphylococcus simulans", Biochemistry Vol.84, 17 October

[4] Gunther Tumm et al, 1997, "Studies on prolysostaphin processing and characterization of the lysostaphin immunity factor (Lif) of Staphylococcus Simulans biovar staphylolyticus", Research Gate, March