Regulatory

Part:BBa_K2143000:Design

Designed by: Bryan Pasqualucci   Group: iGEM16_SCSU-New_Haven   (2016-10-12)


NahR_L154F constitutive transcription factor binds upstream of Psal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 781
    Illegal SapI site found at 1710


Design Notes

This part contains two copies of NahR_L154F. We did this because because we are using it as a positive feedback loop, we wanted the concentration of this mutant to be greater than the concentration of the inducer required allele.


Source

NCBI Reference Sequence: NC_007926.1


References