Composite

Part:BBa_K214001:Design

Designed by: QGEM_09   Group: iGEM09_Queens   (2009-10-19)


Pconst-RBS-Lpp-OmpA-Linker-TEV-TEV-Linker-ANP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 639
    Illegal XhoI site found at 540
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Several restriction sites between domains to allow for future ease of modification.


Source

Combination of biobrick sequences Pconst(BBa_J23119) RBS (BBa_B0034), Lpp (BBa_J36835), OmpA (BBa_J36837), Linker (BBa_K157013-sequence between restriction sites), TEV cut site (BBa_K128002), ANP (consensus mature sequence, Genbank)


References