Composite

Part:BBa_K2139011

Designed by: UBC iGEM 2016   Group: iGEM16_British_Columbia   (2016-10-14)


E1 with Terminator

E1 catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the crystaline cellulose chain. It can be used in conjunction with beta-1,4-exoglucanases and beta-1,4-glucosidase for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature and pH over a wide range (unpublished data). The genbank ascension number for this protein is WP_011719450.1 (amino acid) and a PDB code of 1ECE. This construct was codon optimized for Caulobacter crescentus and synthasized by IDT.

Literature data for this part can be found http://aem.asm.org/content/76/19/6360.full

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None