Coding

Part:BBa_K2136014:Design

Designed by: Livia Seno Ferreira Camargo   Group: iGEM16_USP_UNIFESP-Brazil   (2016-10-13)


Bleomycin resistance gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 774
    Illegal NgoMIV site found at 835
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Introns from the RBCS2 gene were previously added to the native sequence, which increase overall efficiency.

Source

The sequence comes from Streptoalloteichus hindustanus.

References