Regulatory
Part:BBa_K2123105:Design
Designed by: Maria Clara Tavares Astolfi Group: iGEM16_UFAM-UEA_Brazil (2016-10-13)
Stationary phase promoter (PJK26) in tandem + mer operator
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal suffix found in sequence at 152
Illegal EcoRI site found at 2
Illegal XbaI site found at 17 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 2
Illegal SpeI site found at 153
Illegal PstI site found at 167
Illegal NotI site found at 8
Illegal NotI site found at 160 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 2
Illegal BglII site found at 20 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 2
Illegal suffix found in sequence at 153 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 2
Illegal XbaI site found at 17
Illegal SpeI site found at 153
Illegal PstI site found at 167 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Synthesis
Source
Synthesis