Regulatory

Part:BBa_K2123102:Design

Designed by: Maria Clara Tavares Astolfi   Group: iGEM16_UFAM-UEA_Brazil   (2016-10-13)


Tac promoter + overlap mer operator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 68
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 69
    Illegal PstI site found at 83
    Illegal NotI site found at 7
    Illegal NotI site found at 76
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 69
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 69
    Illegal PstI site found at 83
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Synthesis


Source

PO26-CRL

References