Device

Part:BBa_K2122200:Design

Designed by: Sannia Farrukh   Group: iGEM16_NYU-AD   (2016-10-13)


His-tagged Shiga-Like Subunit B toxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1910
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961
    Illegal SapI.rc site found at 2193


Design Notes

Thecode for his-tagged protein was added to both ends of the Gb3 synthase code


Source

Coding Sequence from GenBank

References