Part:BBa_K2122000:Experience

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Applications of BBa_K2122000

The NYU-AD 2016 iGEM team validated BBa_K2122000. The RNA was extracted from the induced colonies and the non-induced colonies as a negative control. RT-PCR was performed and the RNA was converted into DNA. DNA amplification was done by PCR with three sets of forward and reverse primers. Amplified products were un on a 1% agarose gel with sybere green staining and visualised under blue light excitation . Results are in the figure below.

Experimental Validation

The primers used are as follows:
Set 1
Forward: 5- GGA GAA AGG GCA GCT CTA TAA C -3
Reverse: 5- CGG ATG GAA CAC CAC TTC TT -3

Set 2
Forward: 5- CCT GTT CAT CAT CGG CTT CA -3
Reverse: 5- AAG TCC GTG TCC AGG TAG AT -3

Set 3
Forward: 5- ATC TAC CTG GAC ACG GAC TT -3
Reverse: 5- TGG CTC TTC TTG TTC CAC AC -3

User Reviews

UNIQd1a3cd791c9ebd90-partinfo-00000001-QINU UNIQd1a3cd791c9ebd90-partinfo-00000002-QINU