Part:BBa_K2122000:Design

Device expressing the Gb3 Synthase enzyme under control of an arabinose inducible promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1205
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 1144
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1910
  Illegal AgeI site found at 979
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 961
  Illegal SapI.rc site found at 2193
  

Design Notes

An inducible promoter (BBa_10500), RBS (BBa_B0034) Scar was added to the sequence. Along with a terminator.

Source

Coding Sequence from GenBank